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cd81 apc  (Miltenyi Biotec)


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    Miltenyi Biotec cd81 apc
    Cytometry plots and histograms of seminal extracellular vesicles (EVs) characterization. A) Flow cytometry beads of know size (200 nm – 2 µm). B) GFP EVs were used to identify EVs population. C) PBS 1X 0.22 μM filtered. D) Negative control of CellTrace Violet (CTV) staining diluted in PBS. E) High fertility (HF) and reduced fertility (RF) unstained EVs. F) HF CTV-stained EVs. G) RF CTV-stained EVs. H) CTV-stained EVs + human anti <t>CD81-APC</t> antibody (1:50). I) CTV-stained EVs + human anti CD63-PE antibody (1:50). J) CTV-stained EVs + human anti Hsp70-FITC antibody (1:50). K) Lysated EVs with Triton 1X + 0.1% SDS. Photodetectors used: Violet Side Scatter – Height (Violet SSC-H), Blue 525-Area (B525-A), Blue 525-Height (B525-H), Violet 450-Height (V450-H), Blue 585-Height (B585-H) and Red 660-Height (R660-H).
    Cd81 Apc, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 95/100, based on 50 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/cd81+apc/bio_rxiv__64898__2026__03__16__712050-94-40-44?v=Miltenyi+Biotec
    Average 95 stars, based on 50 article reviews
    cd81 apc - by Bioz Stars, 2026-08
    95/100 stars

    Images

    1) Product Images from "Seminal extracellular vesicles from boar AI doses contain fertility-predictive protein and miRNA cargo and improve sperm physiology"

    Article Title: Seminal extracellular vesicles from boar AI doses contain fertility-predictive protein and miRNA cargo and improve sperm physiology

    Journal: bioRxiv

    doi: 10.64898/2026.03.16.712050

    Cytometry plots and histograms of seminal extracellular vesicles (EVs) characterization. A) Flow cytometry beads of know size (200 nm – 2 µm). B) GFP EVs were used to identify EVs population. C) PBS 1X 0.22 μM filtered. D) Negative control of CellTrace Violet (CTV) staining diluted in PBS. E) High fertility (HF) and reduced fertility (RF) unstained EVs. F) HF CTV-stained EVs. G) RF CTV-stained EVs. H) CTV-stained EVs + human anti CD81-APC antibody (1:50). I) CTV-stained EVs + human anti CD63-PE antibody (1:50). J) CTV-stained EVs + human anti Hsp70-FITC antibody (1:50). K) Lysated EVs with Triton 1X + 0.1% SDS. Photodetectors used: Violet Side Scatter – Height (Violet SSC-H), Blue 525-Area (B525-A), Blue 525-Height (B525-H), Violet 450-Height (V450-H), Blue 585-Height (B585-H) and Red 660-Height (R660-H).
    Figure Legend Snippet: Cytometry plots and histograms of seminal extracellular vesicles (EVs) characterization. A) Flow cytometry beads of know size (200 nm – 2 µm). B) GFP EVs were used to identify EVs population. C) PBS 1X 0.22 μM filtered. D) Negative control of CellTrace Violet (CTV) staining diluted in PBS. E) High fertility (HF) and reduced fertility (RF) unstained EVs. F) HF CTV-stained EVs. G) RF CTV-stained EVs. H) CTV-stained EVs + human anti CD81-APC antibody (1:50). I) CTV-stained EVs + human anti CD63-PE antibody (1:50). J) CTV-stained EVs + human anti Hsp70-FITC antibody (1:50). K) Lysated EVs with Triton 1X + 0.1% SDS. Photodetectors used: Violet Side Scatter – Height (Violet SSC-H), Blue 525-Area (B525-A), Blue 525-Height (B525-H), Violet 450-Height (V450-H), Blue 585-Height (B585-H) and Red 660-Height (R660-H).

    Techniques Used: Cytometry, Flow Cytometry, Negative Control, Staining



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    Image Search Results


    Cytometry plots and histograms of seminal extracellular vesicles (EVs) characterization. A) Flow cytometry beads of know size (200 nm – 2 µm). B) GFP EVs were used to identify EVs population. C) PBS 1X 0.22 μM filtered. D) Negative control of CellTrace Violet (CTV) staining diluted in PBS. E) High fertility (HF) and reduced fertility (RF) unstained EVs. F) HF CTV-stained EVs. G) RF CTV-stained EVs. H) CTV-stained EVs + human anti CD81-APC antibody (1:50). I) CTV-stained EVs + human anti CD63-PE antibody (1:50). J) CTV-stained EVs + human anti Hsp70-FITC antibody (1:50). K) Lysated EVs with Triton 1X + 0.1% SDS. Photodetectors used: Violet Side Scatter – Height (Violet SSC-H), Blue 525-Area (B525-A), Blue 525-Height (B525-H), Violet 450-Height (V450-H), Blue 585-Height (B585-H) and Red 660-Height (R660-H).

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    Article Title: Seminal extracellular vesicles from boar AI doses contain fertility-predictive protein and miRNA cargo and improve sperm physiology

    doi: 10.64898/2026.03.16.712050

    Figure Lengend Snippet: Cytometry plots and histograms of seminal extracellular vesicles (EVs) characterization. A) Flow cytometry beads of know size (200 nm – 2 µm). B) GFP EVs were used to identify EVs population. C) PBS 1X 0.22 μM filtered. D) Negative control of CellTrace Violet (CTV) staining diluted in PBS. E) High fertility (HF) and reduced fertility (RF) unstained EVs. F) HF CTV-stained EVs. G) RF CTV-stained EVs. H) CTV-stained EVs + human anti CD81-APC antibody (1:50). I) CTV-stained EVs + human anti CD63-PE antibody (1:50). J) CTV-stained EVs + human anti Hsp70-FITC antibody (1:50). K) Lysated EVs with Triton 1X + 0.1% SDS. Photodetectors used: Violet Side Scatter – Height (Violet SSC-H), Blue 525-Area (B525-A), Blue 525-Height (B525-H), Violet 450-Height (V450-H), Blue 585-Height (B585-H) and Red 660-Height (R660-H).

    Article Snippet: To immunophenotype the EVs, 25 μL of samples (aproximately 2.0 x 10 9 EVs/mL) were incubated 30 min at 38 °C with conjugated antibodies against specific EVs markers: human anti CD63-PE diluted 1:50 (Ref. 130-118-150, Miltenyi Biotec, Germany), human anti CD81-APC 1:50 (Ref. 130-119-825, Miltenyi Biotec) and human anti Hsp70-FITC 1:50 (Ref. 130-124-700, Miltenyi Biotec).

    Techniques: Cytometry, Flow Cytometry, Negative Control, Staining